• Based on the differentiation and siRNA

  2018-10-20

  

  Based on the differentiation and siRNA studies reported here, the mechanistic regulation of FOXM1 towards pluripotency control and differentiation might differ between mouse and human ES cells. The discrepancy can be attributed to the different developmental status of human versus mouse ES cells (Ni

• The karyotype of the BIOT EHMT iPSC line

  2018-10-20

  

  The karyotype of the BIOT-0708-EHMT1 iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). In parallel, the pathogenic NM_024757.4(EHMT1):c.3413G>A mutation was confirmed by Sanger sequencing in the newly established iP

• In our reprogramming culture aside from

  2018-10-20

  

  In our reprogramming culture, aside from insulin production, we also observed expression of α and δ cell hormones, glucagon and somatostatin, respectively in a sub-population of cells. This is likely due to the constitutive expression of reprogramming factors Ngn3 and Pax6. Both transcription factor

• br Material and methods br Results br Discussion Pre

  2018-10-20

  

  Material and methods Results Discussion Pre-transplant myeloablation has been consistently seen to be associated with an increase in the number of adipocytes in the bone marrow (Snyder, 1965; Naveiras et al., 2009; Cao et al., 2011). These marrow adipocytes were considered as passive space

• br Material and methods br Acknowledgement We thank Jane

  2018-10-20

  

  Material and methods Acknowledgement We thank Jane Hagelskjær Knudsen, Christian Knudsen, and Bettina Hansen for excellent technical assistance. This project was supported by Aarhus University and partially by the Lundbeck Foundation (R151-2013-14439) and Danish Council for Independent Researc

• Another major limitation of the adult

  2018-10-20

  

  Another major limitation of the adult cardiac stem cell field is the inability to get full differentiation from putative stem/progenitor rac inhibitor in vitro or in vivo. In fact, although expression of cardiac genes and proteins is demonstrated frequently, the levels, relative to authentic CMs, an

• Unlike these previous methods the

  2018-10-20

  

  Unlike these previous methods, the 3S reprogramming system generates partially reprogrammed iPSCs in a reproducible and predictable manner by regulating the amount of KLF4. These H 89 resume reprogramming upon increased KLF4 expression and therefore are not aberrantly reprogrammed but have paused d

• br Results br Discussion In this study

  2018-10-20

  

  Results Discussion In this study, we integrated transcriptome and proteome data and characterized the inventory of HSPCs and their immediate myeloid committed progeny (LS−K) (Figure S6). With almost 20,000 identified expressed genes including more than 3,000 significantly regulated ones, these

• Pathway analysis on these genes identified their role

  2018-10-20

  

  Pathway analysis on these genes identified their role in stem-cell-maintenance-related Wnt pathway (Baker, 2010), cell-cycle-related p53 pathway, JAK stat, and MAPK pathways in addition to others listed in Table S4. These results suggest a potential contribution of multiple signaling pathways for st

• br Results br Discussion Several strategies have been

  2018-10-20

  

  Results Discussion Several strategies have been adopted to achieve reprogramming, namely, iPSC technology, SCNT, cell fusion, and cell-extract treatment (Gurdon, 2006; Håkelien et al., 2002; Tada et al., 1997; Takahashi and Yamanaka, 2006). Among these approaches, the use of cell extracts has

• Givinostat Here we set out to

  2018-10-20

  

  Here, we set out to investigate the progressive steps of hiPSC reprogramming by Phi29 DNA polymerase-based mRNA-sequencing (Phi29-mRNA amplification [PMA] RNA-seq) that enables us to monitor transcriptomes in scarce intermediate cell populations (Pan et al., 2013). We identified unique pluripotency-

• The genomic stability of hIPSCs has been

  2018-10-20

  

  The genomic stability of hIPSCs has been extensively studied since this could be a major limitation for clinical applications (Aasen et al., 2008). Factors that affect the genomic integrity of stem hcv virus include culture conditions, number of passages, and enzymatic and non-enzymatic dissociatio

• In mouse embryos both the developing retina and

  2018-10-20

  

  In mouse embryos, both the developing retina and the ventral hypothalamus show strong Rax expression (Ikeda et al., 2005; Wataya et al., 2008; Wilson and Houart, 2004). Consistent with previous studies (Ikeda et al., 2005; Wataya et al., 2008), we confirmed that SHH agonists upregulated Rax in mESCs

• While SUMO has not previously been recognized as a

  2018-10-20

  

  While SUMO2 has not previously been recognized as a roadblock to reprogramming, a recent report suggested that knockdown of the upstream SUMO-conjugating enzyme UBC9 blocks iPSC formation (Tahmasebi et al., 2014). Several reasons may account for the apparent discrepancy between our studies. For exam

• In many studies the reprogramming factors were

  2018-10-20

  

  In many studies, the reprogramming factors were delivered individually using monocistronic vectors. However, due to differences in vector uptake, expression levels of each gene in each cell are highly variable (Lo et al., 2015). Since the ratio between the factors is one of most critical factors for

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