In most industrial areas which are
In most industrial areas which are in need of treatment, there are mixtures of different contaminants at different concentrations in soil, underground water or wastewater. These hazardous wastes include a variety of salts, organic matter, heavy metals, trace elements and radioactive compounds .Simultaneous refinement of multiple contaminants using chemical and traditional methods is both technically difficult and costly, while these methods damage soil biotic compounds .Heavy metals such as zinc and copper are necessary for normal growth of plants, however, high concentrations of essential metals also leads to prevent the plant growth and toxicity. The plants tolerance range is to a certain amount and plants can perform detoxification with particular cellular mechanisms of heavy metal . Due to the systemic characteristics of photosynthetic power with a high performance, plants are able to refinement the elements during their numerous metabolic functions. Therefore, the emerging phyto-technology of phytoremediation is highly regarded in the world for refinement of soil, underground water and wastewater contaminants due to being low cost and requiring low technology , . Baycu et al., in their study in 2006 revealed that concentrations of lead, zinc, cadmium and nickel were measured in the leaf of seven species of deciduous trees (Acer negundo L., Aesculus hippocastaneum L., Ailanthus altissima, Fraxinus angustifolia, Acer velutinum L, Populus nigra L, Robinia pseudoacacia L) in urban areas of Istanbul which the highest accumulation amount of cadmium and zinc, lead, and nickel was in Populus nigra, Aesculus hippocastaneum L and Robinia pseudoacacia L, respectively. Lead toxicity in plant none –mind ecotype (NME) Elsholtzia argyi causes the growth inhibition, significant reduction of plant height and root length, reduction of plant wet and dry weight, discoloration of leaves and their folding . The aim of this study is to evaluate ML 239 of zinc in seedlings organs of Acer velutinum spices.
Materials and methods To perform this research, Acer velutinum was selected among the forest species and its two years old seedlings were prepared and transferred to the greenhouse and were held there for thirty days to adapt to the new conditions. Non-contaminated soil (natural soil used in this experiment) was collected from a depth of 0–30cm of one of the nurseries then dried and passed through a 2mm sieve. To prepare the soil contaminated with Zinc, after preparing the soil, the suitable salt of the desired element should have been prepared. Thus, to prepare zinc, zinc chloride salts manufactured by Merk, Germany were used. In this study, the method of spraying on soils with concentrations of zero and 130mg of zinc per liter was used and the needed amount of solution was sprayed on the soil gradually and contaminated quite uniform with the soil and then the pots with one liter volume were filled with it. The Fifty sapling in same age and same size were selected and planted in pots, the pots were kept in the greenhouse and the soil moisture was maintained at capacity limit by weighted method, and irrigation, if necessary, was done with distilled water and after a three-month period of seedlings growth, shoots and roots were harvested and washed with water distilled, then dried in an oven at 70°C in 48h for two times and passed through a 2-mm sieve for analysis. Zinc content in plant samples was prepared after digestion of samples by dry digestion method and atomic absorption device . Chlorophyll measurements were performed using Arnon method.To this end, 0.05g of leaf tissue with 5ml acetone was mixed at a concentration of 80%.The resulting mixture was centrifuged for 10min at 13,000rpm. Involves passage of the extract was separated and measured its volume to 8ml of chlorophyll using spectrophotometer at a wavelength of 645nm and 663nm respectively. Method Omokolo was used for sugar extraction . 0.1g of tissue stem wet with 5ml 80% ethanol was mixed in a porcelain mortar and then 10min in water bath at 70°C was placed. Then, to evaporate the alcohol extracts were obtained at 70°C. To remove chlorophyll a ratio of 1 to 5 chloroform was added for 10min at 10000rpm was placed in a centrifuge. Glucose measurements were performed using Mc Creay . Three milliliters Anthrone extract is added with 100ml of boiling water for 20min and we spectrophotometer at a wavelength of 620nm using a sugar solution is determined.