• Co culture of CDSCs with USSCs revealed regulation of osteo

  2018-10-22

  

  Co-culture of CDSCs with USSCs revealed regulation of osteo- and chondro-associated markers, from which BMP2, BMP4, PTHLH, ACAN and FOXF1 were upregulated and BSP, RUNX2, SOX9, TWIST1 and MSX2 were downregulated after co-culture (Supplementary Fig. 8). Many different transcription factors and regula

• Technically these two antibodies have their

  2018-10-20

  

  Technically, these two Wortmannin cost have their limitations. Both are better suited for immunofluorescent analysis in frozen tissue sections than in formalin-fixed paraffin-embedded tissues. In addition, anti-Lrig1-VU did not work for immunoprecipitation under our experimental conditions. FACS an

• It has been proposed that the beneficial

  2018-10-20

  

  It has been proposed that the beneficial effects of cell-based therapies might be mediated via several direct and indirect mechanisms, including recruitment of endogenous progenitors, differentiation into functional cardiomyocytes and vascular cells, induction of angiogenesis, promotion of perfusion

• br Materials and methods br Resource table Resource details

  2018-10-20

  

  Materials and methods Resource table Resource details This line was derived from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). HCM is a genetic heart disease with a prevalence of up to 1 in 200 people and is characterised by unexplained hypertrophy of the left ventricl

• br Author contributions br Competing

  2018-10-20

  

  Author contributions Competing financial interests Acknowledgments We thank Dr. Sravan K. Goparaju for critical reading our manuscript. This work was supported in part by JSPS KAKENHI (23500446) and the iCeMS exploratory grant for junior investigators to KA. TI was supported in part by the

• br Materials and methods br

  2018-10-20

  

  Materials and methods Results Discussion In this study, we examined the behavior of NPCs in response to CsA using both in vitro and in vivo models. The in vitro and in vivo studies show that CsA effectively increases the size of the NPC population. Given that SVZ-derived neurospheres are co

• Based on the differentiation and siRNA

  2018-10-20

  

  Based on the differentiation and siRNA studies reported here, the mechanistic regulation of FOXM1 towards pluripotency control and differentiation might differ between mouse and human ES cells. The discrepancy can be attributed to the different developmental status of human versus mouse ES cells (Ni

• The karyotype of the BIOT EHMT iPSC line

  2018-10-20

  

  The karyotype of the BIOT-0708-EHMT1 iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). In parallel, the pathogenic NM_024757.4(EHMT1):c.3413G>A mutation was confirmed by Sanger sequencing in the newly established iP

• In our reprogramming culture aside from

  2018-10-20

  

  In our reprogramming culture, aside from insulin production, we also observed expression of α and δ cell hormones, glucagon and somatostatin, respectively in a sub-population of cells. This is likely due to the constitutive expression of reprogramming factors Ngn3 and Pax6. Both transcription factor

• br Material and methods br Results br Discussion Pre

  2018-10-20

  

  Material and methods Results Discussion Pre-transplant myeloablation has been consistently seen to be associated with an increase in the number of adipocytes in the bone marrow (Snyder, 1965; Naveiras et al., 2009; Cao et al., 2011). These marrow adipocytes were considered as passive space

• br Material and methods br Acknowledgement We thank Jane

  2018-10-20

  

  Material and methods Acknowledgement We thank Jane Hagelskjær Knudsen, Christian Knudsen, and Bettina Hansen for excellent technical assistance. This project was supported by Aarhus University and partially by the Lundbeck Foundation (R151-2013-14439) and Danish Council for Independent Researc

• Another major limitation of the adult

  2018-10-20

  

  Another major limitation of the adult cardiac stem cell field is the inability to get full differentiation from putative stem/progenitor rac inhibitor in vitro or in vivo. In fact, although expression of cardiac genes and proteins is demonstrated frequently, the levels, relative to authentic CMs, an

• Unlike these previous methods the

  2018-10-20

  

  Unlike these previous methods, the 3S reprogramming system generates partially reprogrammed iPSCs in a reproducible and predictable manner by regulating the amount of KLF4. These H 89 resume reprogramming upon increased KLF4 expression and therefore are not aberrantly reprogrammed but have paused d

• br Results br Discussion In this study

  2018-10-20

  

  Results Discussion In this study, we integrated transcriptome and proteome data and characterized the inventory of HSPCs and their immediate myeloid committed progeny (LS−K) (Figure S6). With almost 20,000 identified expressed genes including more than 3,000 significantly regulated ones, these

• Pathway analysis on these genes identified their role

  2018-10-20

  

  Pathway analysis on these genes identified their role in stem-cell-maintenance-related Wnt pathway (Baker, 2010), cell-cycle-related p53 pathway, JAK stat, and MAPK pathways in addition to others listed in Table S4. These results suggest a potential contribution of multiple signaling pathways for st

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